CLED Agar (L-Cystine Lactose Electrolyte deficient)

Principle
L-Cystine lactose electrolyte deficient medium composed of peptone, tryptone, meat extract, lactose, L-
Cystine and bromothymol blue. Literature suggests that the Proteous species can be controlled by restricting
the electrolytes and by replacing the mannitol by lactose and sucrose with L-Cystine and bromothymol blue.
Peptone, meat extract and tryptone serve as the source of all essential nutrients such as amino acids,
vitamins, other trace factors. L-Cystine is added as a growth supplement for cystine-dependent coliforms.
Lactose is included as a carbon source and plays a crucial role for selection of lactose fermenting microbes.
Brom Thymol Blue is used as a pH indicator. Organisms capable of fermenting lactose will lower the pH of
medium, result in change the color of the medium from green to yellow. Agar is used as a solidifying agent.
Use: Recommended for cultivating, differentiating and enumerating lactose fermenting bacteria in urine.
Contents*

* Formula adjusted for optimum performance and parameters
#Equivalent of Beef extract