Antibiotic Assay Medium No- 11

Principle
The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of
peptone, pancreatic digest of casein, yeast extract, meat extract, dextrose and agar. Peptone, pancreatic
digest of casein, yeast extract and meat extract provide nitrogen, carbon, vitamins essential nutrients.
Glucose serves as energy source. This medium is formulated in such a way to facilitate diffusion of the
antibiotics for antibiotic assay studies. Media is generally used of testing Neomycin, Erythromycin,
Kanamycin like antibiotics.
Use: Recommended for testing antibiotic potency by Kirby-Bauer (Disk diffusion method) or well diffusion
method. (For Erythromycin, Gentamicin, Neomycin B, Paromomycin, Sisomicin and Netilmicin antibiotics)

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