Antibiotic Assay Medium No- 10

Principle
The media is composed according to the USP, recommended as antibiotic assay medium. It is composed of
pancreatic digest of casein, papaic digest of soybean, sodium chloride, dibasic potassium phosphate,
dextrose and agar. Pancreatic digest of casein and papaic digest of soybean provide nitrogen, carbon and
other essential nutrients. Sodium chloride maintain osmatic equilibrium. Dibasic potassium phosphate resist
change in pH. Dextrose serves as energy source. Agar is solidifying agent. The agar concentration is reduced
for the proper diffusion of the antibiotic. The Polysorbate 80 enhances the penetration of antibiotic to the
cytoplasmic membrane. The AAM-10 used as base agar for the antibiotic assay against the Pseudomonas
aeruginosa and Bordetella bronchiseptia.

Use: Recommended for testing antibiotic potency against Pseudomonas aeruginosa for the antibiotics like
Polymixin B, Carbenicillin, Colismethate, Colistin assay.

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