Principle
The tryptone serves the source of nitrogen, yeast extract serves the source of nitrogen and vitamins, and
sodium chloride balances the osmotic balance. Agar is solidifying agent. The 2xYT agar supports the
vigorous growth of E. coli, necessary for the cultivation of microphages.
Use: Recommended for maintenance and cultivating recombinant strains of Escherichia coli and
propagation of bacteriophage.
Contents*
Ingredients Gram/Litre
Tryptone 16.000
Yeast Extract 10.000
Sodium Chloride 5.000
Agar 15.000
pH at 25˚C 7.0 ±0.2
* Formula adjusted for optimum performance and parameters
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