Modified Soyabean Bile Broth Base

Principle
The composition and performance criteria of this medium are as per the specifications laid down in ISO
1999 Draft ISO/DIS 16654. It can also be isolated from clinical samples. Modified soybean bile broth base
medium is composed of casein peptone, soya peptone, dextrose, bile salts, Dipotassium phosphate and
sodium chloride. Casein and soya peptone provides ample amount of the carbon, nitrogen, essential and non-
essential amino acids, along with the trace minerals. The Bile salts in it inhibit the gram-positive microbes.
Dextrose is a carbohydrate source. Phosphate acts as buffering agent and sodium chloride maintain osmatic
equilibrium. Bile broth base used as selective microbial media for enumeration and detection of the
enterococcus family members.
Use: Recommended for detection, enrichment and confirmation of Escherichia coli O157:H7 from food and
animal feeding stuffs and isolated from clinical samples.
Contents*

* Formula adjusted for optimum performance and parameters