Principle
Malt Extract Broth is recommended for the examination of yeasts and molds in the U.S. Food and Drug
Administration’s Bacteriological Analytical Manual (2005). The media is composed of mycological peptone
and malt extract. Mycological peptone provides nitrogen, vitamins, minerals, amino acids and growth
factors. Malt extract provides an energy source for the growth of microorganisms and provide acidic
environment for the growth of yeasts and molds. The low pH favor’s fungal growth and inhibits
contaminating bacteria and makes it useful for cultivating fungi and aciduric microorganisms. Mycological
peptone rapidly gives a luxuriant growth with typical morphology and pigmentation. Malt Extract Broth
Base has been widely used in the maintenance, isolation and identification of fungi and it is also proposed in
several pharmacopeias as a medium for the control of sterility in pharmaceutical products, though it is
mostly used for comparative morphological studies.
Use: Recommended for the detection, isolation and enumeration of yeasts and molds.
Contents*
Ingredients | Gram/Litre |
Mycological peptone | 3 |
Malt Extract | 17 |
pH at 25˚C | 5.4 ±0.2 |
* Formula adjusted for optimum performance and parameters
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